Quyu Xiaozhong recipe exerts anti-inflammatory effect in acute mastitis by inhibiting TLR4/NF-κB signal pathway

Purpose: To investigate the effect of Quyu Xiaozhong recipe (QYXZR) on acute mastitis induced by Staphylococcus aureus in rats via TLR4/NF-κB signal pathway.
 Methods: The rat model was constructed injecting into fourth pair nipples 5-7 days after parturition. were treated with drugs 12 h injection. Pathological changes mammary tissue determined using hematoxylin-eosin (H&E) staining. Myeloperoxidase (MPO) kit used to evaluate MPO activity. Enzyme-linked immunosorbent assay (ELISA) employed determine levels inflammatory factors, while protein expression TLR4, NF-κB-p65, and IκB-α Western blot.
 Results: QYXZR intervention significantly attenuated pathological damage alleviated aureus-induced mastitis. At same time, reduced factors (p < 0.05). Further molecular mechanistic studies revealed that exerted anti-inflammatory effects inhibiting Conclusion: alleviates caused inhibition pathway, thus, providing scientific theoretical basis for extensive use this traditional Chinese medicine treatment